希望生物翻译高手帮忙哈The regulation of the synthesis by the cytokines interleukin-1 (IL-1) and IL-6 of the positive acute-phase protein 1l-acidglycoprotein (AGP) and of the negative acute-phase protein ac2-HS glycoprotein (AHSG) has been

希望生物翻译高手帮忙哈
The regulation of the synthesis by the cytokines interleukin-1 (IL-1) and IL-6 of the positive acute-phase protein 1l-acid
glycoprotein (AGP) and of the negative acute-phase protein ac2-HS glycoprotein (AHSG) has been studied in a long-term
culture system of the human hepatoma cell line Hep3B. The culture system contained 30 nM-sodium selenite as the only
supplement. This allowed maintenance of the synthesis of the proteins under study at a near steady state for over 3
months. An increase in AGP mRNA and a decrease in AHSG mRNA were observed when cells were treated for two
successive 48 h-periods with monocyte-conditioned medium. A return to basal levels was obtained after cessation of the
cytokine addition. Two further additions of cytokines led to alterations in mRNA levels similar to those observed
following the first cytokine treatment. The amounts of AGP and AHSG secreted were altered in accordance with the
mRNA modifications. These results suggest that new cytokine receptors were being constantly synthesized during cell
culture. When cytokines were present in the culture medium for 10 days, maximum alterations in AGP and AHSG
synthesis were obtained following 2 and 4 days of treatment respectively, but further alterations in protein levels could
not be observed afterwards. Expression of IL-6 receptor mRNA was not up-regulated by cytokines, but only by 1/tMdexamethasone.
Our results show that, in this long-term culture system, cytokines induce a response in hepatoma cells similar to that observed in vivo during human inflammatory states. This model could be used to evaluate the effects of agonists or antagonists of cytokines responsible for the hepatic acute-phase protein response.

该规例的合成是由细胞因子白细胞介素1 （白细胞介素-1 ）和白细胞介素-6的积极急性时相反应蛋白1升酸
糖蛋白（ AGP显卡）和负急性时相反应蛋白ac2 -房协糖蛋白（ ahsg ）一直在研究一个长远的
文化系统的人肝癌细胞系hep3b .文化系统的载为30 nm -亚硒酸钠作为唯一的
补充.这使得维修的合成蛋白质的研究下,在附近的稳定状态,为超过300
个月.增加在AGP接口的mRNA和减少在ahsg mRNA的表达,观察时,细胞治疗为2
连续48小时周期与单核细胞条件培养基.返回基底水平,获得了后,停止该
此外,细胞因子.二,进一步增加了细胞因子,导致改建在mRNA水平类似,以观察
继第一细胞因子治疗.数额AGP和ahsg分泌改变了,按照与
mRNA的修改.这些结果表明,新的细胞因子受体不断被合成在细胞
文化.当细胞因子的人出席,在培养基为10天,最高的改建在AGP和ahsg
合成,获得了以下2和第4天的治疗,但进一步的改动,在蛋白质水平可
没有得到遵守.表达白细胞介素-6受体mRNA的表达没有上调细胞因子,但只有1/tmdexamethasone .
我们的研究结果表明,在这方面长期培养系统,细胞因子诱导的反应,在肝癌细胞相似,观察到在体内在人类炎症国.这个模型可以用来评估的影响激动剂或拮抗剂细胞因子的负责肝急性期蛋白反应.